Destyano, Azel (2026) Pengaruh Konsentrasi Substrat dan Jumlah Bakteri Pada Produksi Biobutanol dari Molase Menggunakan Clostridium Acetobutylicum. Undergraduate thesis, UPN Veteran Jawa Timur.
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Abstract
Molasses, a sugar industry by-product rich in fermentable sugars, has potential as a substrate for biobutanol production. This study investigated the effects of molasses concentration and *Clostridium acetobutylicum* inoculum size on butanol production and identified the optimum fermentation conditions. Molasses concentrations of 3–7% and bacterial inoculum levels of 0.26–1.32 × 10⁹ CFU were evaluated. The pretreated molasses was fermented anaerobically at 37 °C for 4 days with CaCO₃ as a pH buffer. The highest butanol yield was obtained at 6% molasses and 1.32 × 10⁹ CFU, reaching 6.06% by UV–Vis analysis and 3.80% by GC–MS. These results demonstrate that both substrate concentration and inoculum size significantly influence butanol production, confirming the potential of molasses as a feedstock for biobutanol production through anaerobic fermentation.
| Item Type: | Thesis (Undergraduate) | ||||||||
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| Subjects: | T Technology > TP Chemical technology > TP155 Chemical engineering | ||||||||
| Divisions: | Faculty of Engineering > Departement of Chemical Engineering | ||||||||
| Depositing User: | Azel Azel Destyano Destyano | ||||||||
| Date Deposited: | 23 Jul 2026 03:37 | ||||||||
| Last Modified: | 23 Jul 2026 04:09 | ||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/56968 |
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