Yuningsih, - (2026) INDUKSI KETAHANAN TANAMAN BAWANG MERAH (Allium ascalonicum L.) DENGAN Bacillus spp. TERHADAP PENYAKIT MOLER (Fusarium sp.). Masters thesis, UPN Veteran Jawa Timur.
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Abstract
Moler disease caused by Fusarium sp. is one of the major constraints in shallot (Allium ascalonicum L.) cultivation, resulting in significant growth inhibition and yield losses. Disease control still relies heavily on chemical fungicides, which may cause negative impacts on the environment and human health. Therefore, environmentally friendly alternatives are required. This study aimed to evaluate the ability of Bacillus isolates Bcz 14 and Bcz 20 to induce resistance in shallot plants against moler disease, as well as their effects on plant growth and phytochemical compounds. The research was conducted at the Laboratory of Plant Pest and Disease Observation, Madiun, from August to November 2024. Antagonistic activity was evaluated in vitro against Fusarium sp. and in vivo on shallot plants using bacterial populations of 10⁸ CFU/mL. Observed parameters included pathogen inhibition, incubation period, disease intensity, plant growth characteristics (number of leaves, number of tillers, plant height, root length), bulb weight, and phytochemical content. The results showed that in vitro inhibition of Fusarium sp. by Bacillus isolates Bcz 14 and Bcz 20 reached 65.34% and 77.60%, respectively. In vivo application demonstrated that isolate Bcz 20 reduced moler disease intensity by up to 73.91%, which was higher than isolate Bcz 14 (56.51%), and delayed disease incubation by four days. In addition, Bacillus spp. functioned as plant growth promoting rhizobacteria (PGPR) by enhancing vegetative growth and yield components. Phytochemical analysis revealed that Bacillus spp. induced the production of flavonoids and saponins, while Bacillus isolate Bcz 14 induced flavonoids, saponins and tannins. Saponins were identified as the dominant compounds associated with reduced disease intensity. These findings indicate that Bacillus spp., particularly isolate Bcz 20, have strong potential as biological control agents for sustainable shallot cultivation. Keywords: shallot, Bacillus spp., moler disease, Fusarium sp., induced resistance
| Item Type: | Thesis (Masters) | ||||||||||||
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| Subjects: | S Agriculture > S Agriculture (General) | ||||||||||||
| Divisions: | Faculty of Agriculture > Magister Agritechnology | ||||||||||||
| Depositing User: | Yuningsih Yuningsih | ||||||||||||
| Date Deposited: | 12 Jun 2026 08:38 | ||||||||||||
| Last Modified: | 12 Jun 2026 08:38 | ||||||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/53961 |
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