Simanjuntak, William Fernando (2026) Molecular Identification of Symbiotic Bacteria in Entomopathogenic Nematodes Heterorhabditis sp. Undergraduate thesis, UPN Veteran Jawa Timur.
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Abstract
The excessive use of chemical pesticides has caused various negative impacts on the environment and human health, highlighting the need for sustainable biological control alternatives. This study aimed to molecularly identify symbiotic bacteria associated with Heterorhabditis sp. using the 16S rRNA gene approach. Entomopathogenic nematodes were explored from sugarcane plantation soils in Kediri Regency, East Java, using the baiting method, followed by nematode propagation and molecular identification of the symbiotic bacteria. The results showed that the isolated nematodes were identified as Heterorhabditis sp., while the symbiotic bacteria were identified as Chromobacterium rhizoryzae. This bacterium exhibited morphological and physiological characteristics similar to those of the genus Photorhabdus; however, molecular analysis revealed a distant genetic relationship. These findings indicate that Heterorhabditis sp. is not strictly monoxenic but can associate with multiple bacterial species (polyxenic).
| Item Type: | Thesis (Undergraduate) | ||||||||||||
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| Subjects: | S Agriculture > SB Plant culture > SB950-989 Pest Control and Treatment of Diseases, Plant Protection | ||||||||||||
| Divisions: | Faculty of Agriculture > Departement of Agritechnology | ||||||||||||
| Depositing User: | WIlliam Fernando Simanjuntak | ||||||||||||
| Date Deposited: | 05 Feb 2026 07:50 | ||||||||||||
| Last Modified: | 05 Feb 2026 07:50 | ||||||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/49292 |
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