Molecular Identification of Symbiotic Bacteria in Entomopathogenic Nematodes Steinernema spp. Isolates from Kediri Regency

Maulana, Arman Adrian (2025) Molecular Identification of Symbiotic Bacteria in Entomopathogenic Nematodes Steinernema spp. Isolates from Kediri Regency. Undergraduate thesis, UPN Veteran Jawa Timur.

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Abstract

Microorganisms that have been developed as Biological Control Agents (BCAs) include Entomopathogenic Nematodes (EPNs), which generally originate from two families, Heterorhabditidae and Steinernematidae. The infection mechanism of EPNs occurred through the release of their symbiotic bacteria into the host’s hemolymph. Several studies revealed that the symbiotic bacteria of EPNs were not composed of a single species, but instead formed a pathobiome. This study aimed to identify the species of EPNs and their associated bacteria from soil samples collected in Klanderan Village, Plosoklaten District, Kediri Regency. Sampling was conducted using a diagonal pattern and followed by identification procedures. The results showed that the EPN species obtained was Steinernema carpocapsae, based on morphological and morphometric observations. Meanwhile, molecular identification revealed that one of the bacteria associated with Steinernema carpocapsae was Brucella pseudogrignonensis.

Item Type:

Thesis (Undergraduate)

Contributors:

Contribution	Contributors	NIDN/NIDK	Email
Thesis advisor	Kusuma, Ramadhani Mahendra	NIDN0019049304	ramadhani_mahendra.agro@upnjatim.ac.id
Thesis advisor	Rahmadhini, Noni	NIDN00180489905	nonirahmadhini.agrotek@upnjatim.ac.id

Subjects:

S Agriculture > SB Plant culture > SB950-989 Pest Control and Treatment of Diseases, Plant Protection

Divisions:

Faculty of Agriculture > Departement of Agritechnology

Depositing User:

Arman Adrian Maulana

Date Deposited:

22 Dec 2025 01:49

Last Modified:

22 Dec 2025 01:49

URI:

https://repository.upnjatim.ac.id/id/eprint/48444