Pratama, Andhika Wahyu (2025) Efektivitas Bacillus mycoides Ba-11 dan Bacillus sp. Bcz 20 Terhadap Pengendalian Penyakit Hawar Daun Bakteri dan Pertumbuhan Tanaman Padi. Undergraduate thesis, UPN Veteran Jawa Timur.
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Abstract
Bacterial Leaf Blight (BLB) caused by Xanthomonas oryzae pv. oryzae is a major disease of rice that can significantly reduce plant growth and yield. The use of chemical bactericides such as streptomycin sulfate may cause environmental problems; therefore, environmentally friendly alternatives are required. This study aimed to evaluate the effectiveness of Bacillus mycoides Ba-11 and Bacillus sp. Bcz 20 in suppressing bacterial leaf blight disease and promoting rice plant growth. The research consisted of in vitro and in vivo experiments. The in vitro assay used the well diffusion method, while the in vivo experiment employed a factorial Completely Randomized Design with two factors: Bacillus isolates and application doses (20, 25, and 30 ml per plant). The in vitro results showed that Bacillus mycoides Ba-11 and Bacillus sp. Bcz 20 inhibited the growth of Xanthomonas sp. with average inhibition zones of 7.78 mm and 10.39 mm, respectively, with no significant difference between isolates. In vivo tests showed that Bacillus sp. Bcz 20 and a dose of 30 ml/plant produced an effectiveness in controlling bacterial leaf blight disease in rice plants that was classified as less to quite effective with percentages of 40.7% and 41.41%, respectively. This effectiveness value showed an increase of approximately 40% compared to the positive control treatment (streptomycin sulfate bactericide) which showed no control effectiveness (0%). Bacillus application also enhanced plant growth, where treatments with Bacillus mycoides Ba-11, Bacillus sp. Bcz 20, and the 20 ml per plant dose resulted in plant heights of 69.3 cm, 67.2 cm, and 68.3 cm, respectively, compared to the negative control (60.2 cm). The number of tillers produced by Bacillus mycoides Ba-11 and Bacillus sp. Bcz 20 reached 2.16 and 2.18, respectively, higher than the negative control (1.94). These results indicate that both Bacillus isolates have strong potential as biological control agents for bacterial leaf blight while simultaneously enhancing rice plant growth.
| Item Type: | Thesis (Undergraduate) | ||||||||||||
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| Subjects: | S Agriculture > SB Plant culture > SB950-989 Pest Control and Treatment of Diseases, Plant Protection S Agriculture > SB Plant culture > SB599-990.5 Pests and Diseases |
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| Divisions: | Faculty of Agriculture > Departement of Agritechnology | ||||||||||||
| Depositing User: | Andhika Wahyu Pratama | ||||||||||||
| Date Deposited: | 22 Dec 2025 06:25 | ||||||||||||
| Last Modified: | 22 Dec 2025 06:25 | ||||||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/48405 |
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