Kholifah, Wilujeng Rastinur (2025) Kinetika Degradasi Total Petroleum Hidrokarbon Oleh Bakteri Petrofilik Dengan Proses Bioremediasi Pada Lahan Pasca Pertambangan Minyak Dan Gas. Undergraduate thesis, UPN Veteran Jawa Timur.
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Abstract
Oil and gas mining activities can cause soil contamination due to spills and leaks of oil containing total petroleum hydrocarbons (TPH), toxic compounds that can reduce environmental quality. Several remediation methods can be applied to address this contamination, including physical remediation such as electrokinetic treatment, chemical remediation such as soil washing, and bioremediation that utilizes the activity of microorganisms. Bioremediation is considered more efficient, environmentally friendly, economical, and beneficial in restoring soil functions and maintaining ecosystem sustainability in the future. This study aimed to analyze the efficiency of TPH degradation by variations of petrophilic bacteria within 35 days, determine the reaction order and rate constants, and examine the kinetic growth parameters of petrophilic bacteria in the bioremediation process. The treatments used were biostimulation (B0), biostimulation + Pseudomonas fluorescens 12% (B1), biostimulation + Bacillus subtilis 12% (B2), and biostimulation + Pseudomonas fluorescens 6% + Bacillus subtilis 6% (B3). The results of the study showed a degradation efficiency of 45% in variation B0, 60% in variation B1, 76.25% in variation B2, and 77.50% in variation B3 after 35 days. The degradation kinetic analysis indicated that all treatments followed a first-order model, with reaction rate constants of 0.0171 day⁻¹ for B0, 0.0238 day⁻¹ for B1, 0.038 day⁻¹ for B2, and 0.0415 day⁻¹ for B3. The bacterial growth kinetics analysis using the Monod model showed μmax values of 0.785 day⁻¹ and Ks of 7,654.9 mg/kg for B0, μmax of 0.656 day⁻¹ and Ks of 5,280 mg/kg for B1, μmax of 0.753 day⁻¹ and Ks of 7,160 mg/kg for B2, and μmax of 0.761 day⁻¹ and Ks of 13,600 mg/kg for B3. Overall, this study demonstrates that petrophilic bacteria are capable of effectively degrading hydrocarbons and have potential to be applied as a bioremediation strategy for the restoration of TPH-contaminated soils.
| Item Type: | Thesis (Undergraduate) | ||||||||
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| Subjects: | T Technology > TD Environmental technology. Sanitary engineering | ||||||||
| Divisions: | Faculty of Engineering > Departement of Environmental Engineering | ||||||||
| Depositing User: | Rasti Wilujeng Kh | ||||||||
| Date Deposited: | 04 Dec 2025 07:53 | ||||||||
| Last Modified: | 04 Dec 2025 07:53 | ||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/47425 |
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