Sulistyawati, Hanik (2025) KARAKTERISASI FENOTIPE, MOLEKULER DAN UJI VIRULENSI BEBERAPA ISOLAT Metarhizium sp. UNTUK MENGENDALIKAN URET TEBU (Lepidiota stigma) Fabricius. Post-Doctoral thesis, UPN Veteran Jawa Timur.
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Abstract
Lepidiota stigma has become a serious problem in sugarcane cultivation in East Java. Metarhizium anisopliae, as an entomopathogen, is expected to be able to control L. stigma attacks. This study aims to determine the potential of the isolates found, the spore density required to kill larvae, and the species and compounds produced by Metarhizium sp. based on the exploration results that have the potential as entomopathogens against L. stigma. The study was conducted from August 2024 to March 2025 at the Plant Health Laboratory, Faculty of Agriculture, UPN "Veteran" East Java, the Genetics Science Laboratory in Tangerang, and ResearchHub in Malang. The study began by exploring the Metarhizium sp. isolates, which were then identified morphologically. Test the ability of 20 Metarhizium sp. isolates against the death of L. stigma, with the parameters observed being larval mortality at 10 HSA observations. Next, a second-stage virulence test was conducted with spore densities of 10^6 spores/mL, 10^7 spores/mL, and 10^8 spores/mL, using 5 isolates that showed the best mortality against L. stigma in the first-stage virulence test. The parameters observed were L. stigma mortality. The two best isolates were then molecularly identified, and one best isolates was tested for its metabolite compounds. Based on the results of the analysis of variance and DMRT further tests at the 5% level in the virulence test stage 1, isolates M3, M5, M7, M9, and M12 caused 100% mortality during the observation period so that they were the isolates that would be used for the second-stage virulence test. Meanwhile, based on the analysis of variance and DMRT, further tests in the virulence test stage 2, there was no interaction between the isolate source factor and spore density on mortality. The isolate type factor partially had a significant effect on mortality on days 3 to 7, while the spore density factor partially affected mortality on days 3 to 6 and days 8 to 10. Isolate M3 is generally the best isolate with the highest mortality effect and causes mortality since day 2 and causes higher daily mortality compared to other treatments. While in the spore density factor, the density level of 10^8 spores/ml produces significantly higher mortality compared to other treatments. Isolates M3 and M7 were tested molecularly, and it was found that the Metarhizium sp. identified from the exploration results was M. anisopliae. Additionally, the metabolomic test of secondary metabolite compounds revealed that the Metarhizium sp. from the exploration results contained destruxin E, A4, and Ed1. Destruxin E is the most crucial role in killing insects related to its effect as an antifeedant, disrupting the immune system, and inducing paralysis and muscle contractions in insects. Keywords: L. stigma, entomopathogen, PCR, metabolite compounds
| Item Type: | Thesis (Post-Doctoral) | ||||||||||||
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| Subjects: | S Agriculture > SB Plant culture > SB599-990.5 Pests and Diseases | ||||||||||||
| Divisions: | Faculty of Agriculture > Magister Agritechnology | ||||||||||||
| Depositing User: | Hanik Sulistyawati Sulistyawati | ||||||||||||
| Date Deposited: | 14 Aug 2025 07:35 | ||||||||||||
| Last Modified: | 14 Aug 2025 07:35 | ||||||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/41696 |
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