Azzahra, Fatimah (2025) Optimasi Kadar Glukosa Dari Limbah Cair Hasil Regenerasi Saccharomyces Cerevisiae Dengan Proses Hidrolisis Menggunakan Metode Response Surface Methodology. Undergraduate thesis, UPN Veteran Jawa Timur.
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Abstract
This study examines the optimization of glucose levels from the liquid waste of Saccharomyces cerevisiae regeneration through hydrolysis using sulfuric acid (H₂SO₄) as a catalyst, employing Response Surface Methodology (RSM). The independent variables include waste volume (300–1500 mL) and water volume (200–1000 mL), with fixed conditions of temperature at 30°C, time at 180 minutes, and stirring at 200 rpm. The highest spectrophotometric measurement result reached 29.7% at a waste volume of 900 mL and water volume of 600 mL. Optimization using Minitab 19, based on 13 experimental data points, yielded an optimal glucose content of 22.83% at a waste volume of 1378.79 mL and water volume of 609.35 mL, with a desirability value of 0.929 and an R² of 95.57%. This research demonstrates that the hydrolysis process of liquid waste from Saccharomyces cerevisiae regeneration can be utilized to produce glucose as a value-added raw material, such as for bioethanol production.
| Item Type: | Thesis (Undergraduate) | ||||||||
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| Subjects: | T Technology > TP Chemical technology > TP155 Chemical engineering | ||||||||
| Divisions: | Faculty of Engineering > Departement of Chemical Engineering | ||||||||
| Depositing User: | Fatimah Azzahra - | ||||||||
| Date Deposited: | 04 Nov 2025 02:24 | ||||||||
| Last Modified: | 04 Nov 2025 02:24 | ||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/41353 |
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