Wicaksono, Ferdianto Eko (2024) POTENSI Streptomyces spp. DARI TANAH TANAMAN KACANG TANAH SEBAGAI ENTOMOPATOGEN HAMA Spodoptera litura. Masters thesis, UPN VETERAN JAWA TIMUR.
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Abstract
Peanuts are an agricultural commodity that has a fairly high economic value and plays an important role in meeting human needs, namely as a source of vegetable protein, oil and other nutrients (Saleh, 2010), but domestic peanut production has not been able to meet the needs of the Indonesian people. One of the pests in peanut plants is the armyworm (S. litura). Leaf damage by armyworms can interfere with the photosynthesis process and eventually result in crop loss. The magnitude of yield loss depends on the degree of leaf damage and the stage of plant growth when the infestation occurs. Leaf damage of 12.5% can lead to economic losses equivalent to the cost of twice the application of insecticides. Armyworm (S.litura) is a polyphagous pest caterpillar because the caterpillar can eat all types of plants for its survival and is difficult to control (Sari et al., 2012). This research was conducted from May 2023 to December 2023 Soil Sampling Preparation Activities in Gunung Dulang Village, Klakah District, Lumajang Regency, Biological Agency Isolation, Biological Agency Propagation at the Plant Health Laboratory, Faculty of Agriculture, National Development University "Veteran" East Java and Quality Control Laboratory. This study used Factorial Group Randomized Design (RAK) with 3 factors. The first factor is the addition of the biological agency Streptomyces sp. On the soil before planting and after planting. The second factor of application time will be carried out before the infestation and after the infestation, pest infestation is carried out when peanut plants have begun to enter the generative period (40 HST). The third factor is the concentration of biological agency Streptomyces sp. which will be given as much as 25%, 50%, and 75%, with codes K1, K2, K3, and K0 (0 ml) as controls. Aquadest will be used as a solvent The number of experimental units used is 48 plants where per polybag will be planted 3 peanuts with 3 repeats. PCR Results Based on the data of the BLAST® results tabulation that the isolate belongs to the genus Streptomyces sp. with an identity percentage of 99.86% with Streptomyces sp. VEL gene 17 for 16 rRNA, partial sequence with access code AB914463.2 Streptomyces sp. Irregular and branched coccus-shaped colonies were obtained. The colonies are tightly attached to the GNA medium, wavy white in color with a notched surface and a floury texture. The highest mortality was in the treatment (S0 RP K3) and (S1 RP K3) there was 60%, this shows that with the administration of Streptomyces sp. With a concentration of 75% can cause very high mortality with stomach poison in S. Litura pests . and the LC50 mortality result of S. Litura with a mortality percentage probability of 80.94%. LC50 calculations show 80% results in controlling 50% of S. Litura pests. Streptomyces sp. Has the ability as a biocide that can be used as pest control.
| Item Type: | Thesis (Masters) | ||||||||||||
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| Subjects: | S Agriculture > SB Plant culture > SB950-989 Pest Control and Treatment of Diseases, Plant Protection S Agriculture > S Agriculture (General) |
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| Divisions: | Faculty of Agriculture > Departement of Agritechnology | ||||||||||||
| Depositing User: | FERDIANTO EKO WICAKSONO | ||||||||||||
| Date Deposited: | 10 Jun 2024 05:02 | ||||||||||||
| Last Modified: | 10 Jun 2024 05:02 | ||||||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/24405 |
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