Oktavia, Nanda (2022) Pengaruh Konsentrasi Enzim Chitosanase dan Waktu Inkubasi Terhadap Karakteristik Chitooligosakarida (COS) dan Aktivitas Prebiotik dari Cangkang Kepiting Bakau. Undergraduate thesis, UPN "Veteran" Jawa Timur.
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Abstract
Mangrove crab shell is one of the wastes that is not used properly because it is usually only used as a mixture of animal feed. Mangrove crab shells contain a high enough chitin content, so that they can be used as chitosan and then used as chitooligosaccharides which have the potential to have prebiotic activity. This study aims to determine the effect of chitosanase enzyme concentration and incubation time on chitooligosaccharides' characteristics and prebiotic activity. This study used a completely randomized design with two factors and two replications. Factor I was chitosanase enzyme concentrations of 0.5%, 1%, and 1.5%, and Factor II was incubation times of 1 hour, 3 hours, and 5 hours. Observational data were analyzed using Analysis of Variance (ANOVA), if there was a significant interaction or effect on the two treatments, a 5% DMRT further test was performed. The results showed that the best treatment was chitosanase enzyme concentration of 0.5% and incubation time of 5 hours, which resulted in chitooligosaccharides with the characteristics: the yield of 8.64%, the viscosity of 2.75 cPs, the solubility of 93.5%, degree of deacetylation 97.68%, molecular weight 546.99 Da, and degree of polymerization 2.67. In the best treatment, the prebiotic activity was tested at 1.18 for Lactobacillus acidophilus and 1.33 for Bifidobacterium breve. Keywords: chitosanase enzyme, chitooligosaccharide, prebiotic activity
| Item Type: | Thesis (Undergraduate) | ||||||||||||
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| Subjects: | T Technology > TP Chemical technology > TP368 Food processing and manufacture | ||||||||||||
| Divisions: | Faculty of Engineering > Departement of Food Engineering | ||||||||||||
| Depositing User: | NANDA OKTAVIA | ||||||||||||
| Date Deposited: | 02 Jun 2022 07:43 | ||||||||||||
| Last Modified: | 02 Jun 2022 07:43 | ||||||||||||
| URI: | http://repository.upnjatim.ac.id/id/eprint/6620 |
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Pengaruh Konsentrasi Enzim Chitosanase dan Waktu Inkubasi Terhadap Karakteristik Chitooligosakarida (COS) dan Aktivitas Prebiotik dari Cangkang Kepiting Bakau. (deposited 16 Jun 2022 01:37)
- Pengaruh Konsentrasi Enzim Chitosanase dan Waktu Inkubasi Terhadap Karakteristik Chitooligosakarida (COS) dan Aktivitas Prebiotik dari Cangkang Kepiting Bakau. (deposited 02 Jun 2022 07:43) [Currently Displayed]
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