Afida, Nurul (2025) Potential of Secondary Metabolites of Endophytic Bacteria Bacillus sp. Bth-22 as an Antifungal Agent Against Colletotrichum sp. on Chili Seeds (Capsicum frutescens L.). Undergraduate thesis, UPN Veteran Jawa Timur.
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Abstract
Chili (Capsicum frutescens L.) is a horticultural commodity with high economic value. One of the major constraints in its production is seed-borne pathogens, particularly those caused by the fungus Colletotrichum sp. An alternative control strategy that can be applied is the utilization of secondary metabolites from Bacillus sp., as they possess the ability to produce antifungal compounds. This study aimed to determine the effective concentration levels of secondary metabolites of Bacillus sp. Bth-22 in suppressing Colletotrichum sp. infection on chili seeds, as well as to evaluate their effects on seed germination and plant growth using blotter test and growing-on test methods. The experiment was arranged in a completely randomized design (CRD) with a single factor consisting of various concentrations (10%, 15%, 20%, 25%, and 30%), a negative control (pathogen suspension), and a positive control (chemical fungicide propineb). The results indicated that the optimal concentration of Bacillus sp. secondary metabolites was 30%, which suppressed Colletotrichum sp. infection on seeds by 47.6% in the blotter test and 91.6% in the growing-on test. Furthermore, the 30% concentration also enhanced germination by 64% in the blotter test and 94.3% in the growing-on test. In addition, the 30% concentration increased plant height by 133% compared to the negative control and 7% compared to the positive control, and improved root length by 140% compared to the negative control and 13% compared to the positive control. Keywords: Chili seed, seed treatment, Bacillus sp., Colletotrichum sp., secondary metabolites
| Item Type: | Thesis (Undergraduate) | ||||||||||||
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| Subjects: | S Agriculture > S Agriculture (General) | ||||||||||||
| Divisions: | Faculty of Agriculture > Departement of Agritechnology | ||||||||||||
| Depositing User: | Nurul Afida | ||||||||||||
| Date Deposited: | 17 Sep 2025 08:07 | ||||||||||||
| Last Modified: | 17 Sep 2025 08:07 | ||||||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/43769 |
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