Ameliya, Echa Raniaputri (2025) Pembuatan Asam Buatanoat dari Campuran Sagu dan Tetes Tebu Menggunakan Proses Fermentasi Bakteri Clostridium Acetobutylicum. Undergraduate thesis, UPN Veteran Jawa Timur.
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Abstract
Butanoic acid was produced through anaerobic fermentation using Clostridium acetobutylicum with a mixed substrate of sago starch hydrolysate and molasses. Sago starch was enzymatically hydrolyzed with α-amylase to release glucose, then combined with molasses as an additional source of sugars, vitamins, and minerals. Fermentation was conducted at 37 °C under anaerobic conditions with varying sago-to-molasses ratios (1:3, 1:2, 1:1, 2:1, and 3:1) over 24–120 h. Gas chromatography–mass spectrometry (GC–MS) analysis showed that the 3:1 ratio produced the highest butanoic acid concentration of 22.97% after 120 h without shifting to the solventogenic phase. In contrast, the 1:1 ratio reached an earlier peak of 17.89% at 24 h but decreased due to metabolic transition to solventogenesis. Higher glucose availability enhanced fermentation performance, while higher molasses content increased viscosity and slowed microbial metabolism. These findings highlight that substrate ratio and fermentation duration play a critical role in optimizing butanoic acid yield. The 3:1 ratio was identified as the most effective condition, emphasizing the importance of controlling pH, viscosity, and substrate composition to improve fermentation efficiency. This study demonstrates the potential of local biomass resources for sustainable biofuel development. Keywords: butanoic acid, anaerobic fermentation, Clostridium acetobutylicum, sago starch hydrolysate, molasses, substrate ratio, biofuel.
| Item Type: | Thesis (Undergraduate) | ||||||||
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| Subjects: | Q Science > Q Science (General) Q Science > QA Mathematics Q Science > QC Physics Q Science > QD Chemistry Q Science > QK Botany Q Science > QR Microbiology |
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| Divisions: | Faculty of Engineering > Departement of Chemical Engineering | ||||||||
| Depositing User: | Echa Raniaputri Ameliya | ||||||||
| Date Deposited: | 15 Sep 2025 03:12 | ||||||||
| Last Modified: | 15 Sep 2025 03:12 | ||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/43322 |
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