Yuliana, Septi Lilik (2022) Aktivitas Antimikroba dan Antikolestrol Chitooligosakarida Cangkang Rajungan (Portunus pelagicus). Undergraduate thesis, UPN Veteran Jawa Timur.
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Abstract
The shell of the crab (Portunus pelagicus) is one of the main sources of chitooligosaccharides because it contains 20-30% chitin. Chitin can be modified into chitooligosaccharides through transformation into chitosan and then chitosan is hydrolyzed into chitooligosaccharides. Chitooligosaccharides (COS) are glycoprotein complex compounds derived from chitosan having -1,4 glucosidic bonds with chain lengths of 20 or less. Enzymatic hydrolysis of chitooligosaccharides can be carried out using the chitosanase enzyme. This study aims to determine the optimum conditions for hydrolysis of chitooligosaccharides using the enzyme chitosanase from Bacillus sp with variable enzyme concentration, hydrolysis temperature and hydrolysis time. The results of this study indicate the optimum conditions at 2% enzyme concentration, hydrolysis temperature of 50oC and hydrolysis time of 5 hours. Chitooligosaccharides produced under optimum conditions were then assessed for their antimicrobial activity against Staphylococus aureus and Escherichia coli and decreased cholesterol levels in vitro. This research was conducted using a completely randomized design (CRD) method to optimize hydrolysis conditions and anticholesterol activity. Independent Sample t-Test method for the antimicrobial activity of the Disc Diffusion method. Paired Sample t-Test method for the antimicrobial activity of the Minimum Inhibitory Concentration (MIC) method. Factors for antimicrobial activity used chitooligosaccharide concentrations of 50% and 100% for disc diffusion and chitooligosaccharide concentrations of 6.5%, 12.5%, 25%, 50% and 100% for the MIC method. In testing the anticholesterol activity using the concentration factor of chitooligosaccharides (1%, 2%, 3%, 4%) against cholesterol solution in vitro CHOD-PAP method. The results showed that the optimum hydrolysis conditions were 2% enzyme concentration, temperature 50oC and hydrolysis duration of 5 hours produced chitooligosaccharides which had the characteristics of yield of 69.98% (v/v), reducing sugar content of 8.49 ± 0.38 (mg/v). mL), the degree of deacetylation was 87.62% and the molecular weight was 4.51 KDa. Chitooligosaccharides had better antimicrobial activity on Escherichia coli bacteria than Staphylococcus aureus with a large diameter of inhibition area (DDH) of Escherichia coli of 7.3 ± 0.57 mm and Staphylococcus aureus of 2.4 ± 0.57 mm. Chitooligosaccharides had a Minimum Inhibitory Concentration (MIC) value of Escherichia coli at a concentration of 50%, while Staphylococcus aureus had a chitooligosaccharide concentration of 100%. Chitooligosaccharide concentration of 4% to the cholesterol solution can reduce cholesterol levels by 7.48% from the negative control. Keywords: Anticholesterol, Antimicrobial, Chitooligosaccharide, Chitosanase Enzyme, Optimization
| Item Type: | Thesis (Undergraduate) | ||||||||||||
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| Subjects: | T Technology > TP Chemical technology > TP368 Food processing and manufacture | ||||||||||||
| Divisions: | Faculty of Engineering > Departement of Food Engineering | ||||||||||||
| Depositing User: | Septi Lilik Yuliana | ||||||||||||
| Date Deposited: | 14 Jan 2022 02:33 | ||||||||||||
| Last Modified: | 14 Jan 2022 02:33 | ||||||||||||
| URI: | http://repository.upnjatim.ac.id/id/eprint/4095 |
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