sukendah, Sukendah and Hugo, Volkaerd and Sudarsono, Sudarsono (2017) Isolation and Analysis of Sucrose Synthase (SUS) Gene Fragment Originated from“Kopyor” Coconut Mutant. Isolation and Analysis of Sucrose Synthase (SUS) Gene Fragment Originated from“Kopyor” Coconut Mutant, 23 (12). pp. 12223-1226. ISSN 12223–12226
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Abstract
“Kopyor” coconut is a naturally occurring coconut mutant having abnormal endosperm. The content of sucrose in the “Kopyor” coconut endosperm is twice as much as normal one. Fragment of sucrose synthase (SUS) gene, one coding for sucrose synthase enzyme involved in the sucrose biosynthesis, was isolated from “Kopyor” coconut and its sequence were analyzed. The size of DNA fragments of SUS gene originated from “Kopyor” coconut (CnSus1A, CnSus1B, CnSus2A, and CnSus2B) were 746, 738, 780, and 687 bp, respectively. Analysis of DNA sequences of CnSus1A, CnSus1B, CnSus2A, and CnSus2B showed features of non-grass monocotyl of SUS gene. Gene specific primer pairs (CnSUS1A, CnSUS1B, CnSUS2A, and CnSUS2B) were designed based on sequences of SUS gene fragments and they could be used for analyzing variability of SUS gene among normal and mutant coconut.
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) > S590-599.9 Soils. Soil science |
| Divisions: | Faculty of Agriculture > Departement of Agritechnology |
| Depositing User: | Prof. Dr. Sukendah Sukendah |
| Date Deposited: | 02 May 2023 00:32 |
| Last Modified: | 02 May 2023 00:32 |
| URI: | http://repository.upnjatim.ac.id/id/eprint/13123 |
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