Nadiva, Salma Alfiani (2026) Potensi Isolat Bacillus spp. sebagai Agensia Pengendali Hayati Rhizoctonia solani Penyebab Penyakit Busuk Pelepah Tanaman Jagung (Zea mays L.). Undergraduate thesis, UPN Veteran Jawa Timur.
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Abstract
Corn (Zea mays L.) is one of the leading commodities in Indonesia that plays an important role in supporting national food security. Corn productivity may decrease due to sheath blight disease caused by Rhizoctonia solani.Environmentally friendly control using biological agents such as Bacillus spp. can be an alternative method to suppress disease development. This study aimed to obtain information regarding the effectiveness of laboratory collection isolates of Bacillus spp. against R. solani, the causal agent of corn sheath blight, particularly regarding differences in application timing, which is still limited. The study was conducted through both in vitro and in vivo experiments. The in vitro test used the dual culture method on five Bacillus spp. isolates, namely Bcz-14, Bcz-16, Bcz-20, Bcz-21, and Bcz-30, to determine their inhibitory ability against R. solani. The results showed that all isolates were able to inhibit pathogen growth with varying percentages of inhibition. Isolate Bcz-14 had the highest average inhibitory effect, while isolates Bcz-16 and Bcz-21 were selected for the in vivo test based on the combination of average inhibitory effect and stability of results among replications. The in vivo test was conducted using the two selected isolates combined with application timing at seven days before planting and at planting time. The results showed that treatment B1T1 (Bcz-16 applied seven days before planting) tended to be the best treatment in suppressing disease development, indicated by a longer incubation period and the lowest disease intensity of 17.78%. Isolate Bcz-16 has the potential to be developed as a biological control agent candidate for managing corn sheath blight disease; however, further testing under field conditions is still required. Keywords: Bacillus spp., Rhizoctonia solani, biological control agent.
| Item Type: | Thesis (Undergraduate) | ||||||||||||
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| Subjects: | S Agriculture > SB Plant culture > SB950-989 Pest Control and Treatment of Diseases, Plant Protection S Agriculture > S Agriculture (General) |
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| Divisions: | Faculty of Agriculture > Departement of Agritechnology | ||||||||||||
| Depositing User: | Salma Alfiani Nadiva | ||||||||||||
| Date Deposited: | 03 Jun 2026 04:22 | ||||||||||||
| Last Modified: | 03 Jun 2026 04:22 | ||||||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/53570 |
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