Adhyasta, Ahmad Davin Najwan (2027) POTENTIAL OF A CONSORTIUM OF Bacillus sp., Pseudomonad fluorescent, AND Trichoderma sp. IN ALTERNATIVE SOYBEAN MEDIA ON Ralstonia solanacearum AND TOMATO (Solanum lycopersicum L.) PLANT GROWTH. Undergraduate thesis, UPN "Veteran" Jawa Timur.
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Abstract
Bacterial wilt disease caused by Ralstonia solanacearum is a major constraint in tomato (Solanum lycopersicum L.) cultivation in Indonesia, causing losses of up to 100%. Biological control using antagonistic microbes such as Bacillus sp., fluorescent Pseudomonad, and Trichoderma sp. is an environmentally friendly alternative. This study aimed to test the potential of a consortium of these three microbes in a soybean alternative medium against R. solanacearum and tomato plant growth. The research was conducted in the Screenhouse and Plant Health Laboratory of UPN "Veteran" East Java from November 2024 to July 2025 using In Vitro and In Vivo testing methods with a Completely Randomized Design (CRD). In Vitro testing included compatibility and antagonism, while In Vivo testing observed the incubation period, disease intensity, and agronomic aspects. The In Vitro test results showed that the consortium of Trichoderma sp., Bacillus sp., and fluorescent Pseudomonad (TBP) was compatible in the soybean alternative medium (Compatibility Index 0.873) and had the highest inhibitory effect against R. solanacearum with a clear zone area of 17.2 mm, which was categorized as strong. The inhibition mechanism was classified as bacteriostatic. Furthermore, the In Vivo test results confirmed the optimal effectiveness of the TBP consortium. This consortium treatment provided the longest bacterial wilt disease incubation period, reaching 18.38 days, as well as the lowest and most consistent disease incidence, at 17.15 at 35 DAI (Days After Inoculation). In addition to suppressing the disease, the TBP consortium also significantly improved the agronomic aspects, demonstrated by the highest plant height of 58.54cm, the highest number of leaves of 62.77, and the highest fruit fresh weight of 136.00 grams. In conclusion, the consortium of Trichoderma sp., Bacillus sp., and fluorescent Pseudomonad cultured on a soybean alternative medium has high potential as a biocontrol agent to suppress R. solanacearum bacterial wilt disease while increasing the growth and production of tomato plants.
| Item Type: | Thesis (Undergraduate) | ||||||||||||
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| Subjects: | S Agriculture > SB Plant culture > SB950-989 Pest Control and Treatment of Diseases, Plant Protection S Agriculture > SB Plant culture > SB599-990.5 Pests and Diseases |
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| Divisions: | Faculty of Agriculture > Departement of Agritechnology | ||||||||||||
| Depositing User: | Ahmad Davin Najwan Adhyasta | ||||||||||||
| Date Deposited: | 10 Dec 2025 07:32 | ||||||||||||
| Last Modified: | 10 Dec 2025 07:32 | ||||||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/48318 |
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