Nailufar, Nely (2025) DETEKSI DINI JAMUR Pyricularia oryzae PADA BENIH PADI (Oryza sativa L.) BERBASIS CITRA INFRAMERAH. Undergraduate thesis, UPN "Veteran" Jawa Timur.
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Abstract
Pyricularia oryzae is a seed-borne pathogen that causes blast disease and has the potential to reduce the quality and viability of rice seeds. Early detection of infection in seeds is essential to minimize the risk of pathogen dissemination into cultivation areas. This study aims to analyze the effectiveness of infrared imaging as an early detection method for P. oryzae infection in rice seeds (Oryza sativa L.) and to compare its performance with conventional observation methods. The research was conducted through several stages, including random sampling based on ISTA standards, sterilization, pathogen isolation and spore suspension preparation, seed inoculation, and seed health testing using the blotter test method. Observations were carried out for seven days, involving infrared image acquisition using a digital microscope equipped with an infrared lens, preprocessing (grayscaling), object segmentation, threshold determination, pseudo coloring, and validation of imaging results through regression analysis. The findings indicate that infrared imaging can detect early physiological changes in seeds faster than visual observation. Initial symptoms of infection were identified on day three through pseudo-colored images, indicated by the appearance of yellowish-green coloration on the seed margins, whereas visual symptoms emerged on day four, corresponding to the incubation period of P. oryzae. By days six and seven, the areas of discoloration expanded, indicating increased infection severity. Regression validation produced an R² value of 0.7033, showing that 70.33% of the variation in laboratory observations could be explained by image estimation. These results demonstrate that infrared imaging possesses good accuracy and can serve as a non-destructive, faster, and more sensitive alternative for early detection of P. oryzae infection compared to conventional methods. Keywords: blas disease, preprosecing, thresholding, pseudo coloring
| Item Type: | Thesis (Undergraduate) | ||||||||||||
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| Subjects: | S Agriculture > SB Plant culture > SB950-989 Pest Control and Treatment of Diseases, Plant Protection | ||||||||||||
| Divisions: | Faculty of Agriculture > Departement of Agritechnology | ||||||||||||
| Depositing User: | Nely Nailufar | ||||||||||||
| Date Deposited: | 09 Dec 2025 06:43 | ||||||||||||
| Last Modified: | 09 Dec 2025 06:43 | ||||||||||||
| URI: | https://repository.upnjatim.ac.id/id/eprint/48294 |
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