Compatibility Of Bacillus Sp. Bth-22 and Trichoderma Sp. for Controlling Bacterial Leaf Blight Disease in Rice Plants

Permata, Aprilla Hendiana Dwirizki (2025) Compatibility Of Bacillus Sp. Bth-22 and Trichoderma Sp. for Controlling Bacterial Leaf Blight Disease in Rice Plants. Undergraduate thesis, UPN Veteran Jawa Timur.

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Abstract

Bacterial leaf blight (BLB), caused by Xanthomonas sp., is one of the most destructive diseases affecting rice plants in Indonesia. This study aimed to evaluate the compatibility and antagonistic effectiveness of Bacillus sp. Bth-22 and Trichoderma sp. applied both individually and in consortium against Xanthomonas sp. through in vitro and in vivo experiments. The research was conducted at the Plant Health Laboratory of the Faculty of Agriculture, UPN “Veteran” East Java and Kebun Bibit’s Greenhouse from February to July 2025. The in vitro test, using the dual culture method, revealed that Bacillus sp. Bth-22 and Trichoderma sp. were compatible, indicated by the absence of inhibition zones and a compatibility index below 1. Antagonism tests showed that the consortium treatment produced the highest inhibition zone (13 mm, strong category), significantly greater than single treatments of Bacillus sp. (1 mm) and Trichoderma sp. (8.3 mm). In vivo results demonstrated that the consortium effectively reduced disease intensity to the lowest level (30.02%) at 55 days after planting, compared to control treatments. Additionally, plants treated with antagonistic microbes exhibited increased height and leaf number, indicating improved growth. These findings confirm that the combination of Bacillus sp. Bth-22 and Trichoderma sp. works synergistically to suppress Xanthomonas sp. infection and enhance rice growth, suggesting its potential as a sustainable biocontrol strategy for managing bacterial leaf blight in rice.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorPurnawati, ArikaNIDN0022046505arika_p@upnjatim.ac.id
Thesis advisorPrasetyawati, Endang TriwahyuNIDN0703126403endang_tp@upnjatim.ac.id
Subjects: S Agriculture > SB Plant culture > SB950-989 Pest Control and Treatment of Diseases, Plant Protection
S Agriculture > S Agriculture (General)
S Agriculture > S Agriculture (General) > S631-667 Fertilizers and Improvement of the soil
S Agriculture > SB Plant culture
S Agriculture > SB Plant culture > SB599-990.5 Pests and Diseases
Divisions: Faculty of Agriculture > Departement of Agritechnology
Depositing User: Aprilla Hendiana Dwirizki Permata
Date Deposited: 23 Oct 2025 06:48
Last Modified: 23 Oct 2025 06:48
URI: https://repository.upnjatim.ac.id/id/eprint/45679

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