Ummah, Robithotul (2023) UJI KOMPATIBILITAS DAN UJI FITOKIMIA BIOAKTIVATOR Trichoderma harzianum DARI LAHAN BAWANG MERAH MINIM DAN INTENSIF FUNGISIDA TERHADAP PATOGEN Alternaria sp. Masters thesis, UPN Veteran Jawa Timur.
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Abstract
Shallots (Allium ascalonicum L.) are one of the main vegetable commodities in Indonesia. Shallot productivity fluctuates due to various factors, especially purple spot disease. In the Denanyar area, Jombang there is a problem of purple spot onion disease caused by the pathogenic fungus Alternaria sp. This pathogen can cause yield losses of up to 55% and will continue to increase in the rainy season. The aim of this research is to study the antibiosis mechanism of T. harzianum in controlling Alternaria sp., to study the dosage of fungicides containing the active ingredient mancozeb which are compatible with T. harzianum, as well as the secondary metabolite compounds produced by T. harzianum from minim and intensive fields of fungicides containing the active ingredient mancozeb through phytochemical tests. This research was conducted at the Agricultural Laboratory of KH. A. Wahab University in Jombang and collecting isolates in Denayar village, Jombang. The research period starts from July 2023 to November 2023. The research method has 3 stages, namely the initial stage is preparation, stage 1 is research, and stage 2 is research. This research was carried out using a Completely Randomized Design (CRD) method with treatment according to the test. The variables observed were the growth rate of Alternaria sp., growth inhibition of Alternaria sp., inhibitory power of T. harzianum against Alternaria sp., compatibility of T. harzianum with fungicides containing the active ingredient mancozeb, and phytochemical compounds of alkaloids, flavonoids, steroids, tannins and saponins. The analysis results of the observed variables are processed using the Anova test, if there are significantly different values then they are further tested using the DMRT test (Duncan Multiple Range Test) at an error level of 5%. The results of the research show that the working mechanism of the T. harzianum bioactivator is antibiosis which produces secondary metabolite compounds in controlling the pathogen Alternaria sp. The lowest percentage of T. harzianum inhibition was at 3 days, namely the TM1 treatment at 34.62% and the highest at the TI5 treatment at 77.48%. At 7 days the lowest inhibition in the TM1 treatment was 0.7% and the highest in the TI5 treatment was 57.8%. The secondary metabolite compounds produced by T. harzianum from minim and intensive fungicide fields positively contain steroid compounds, alkaloids, flavonoids, tannins and saponins. In the GCMS test the compounds with the highest counter values were Dodecane, 2,7,10-trimethyl- (C15H32), Hexadecane, 2,6,11,15-tetramet (C20H42), Eicosane, 2-methyl- (C21H44), and Heneicosane (C21H44).
| Item Type: | Thesis (Masters) | ||||||||||||
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| Subjects: | S Agriculture > S Agriculture (General) | ||||||||||||
| Divisions: | Faculty of Agriculture > Magister Agritechnology | ||||||||||||
| Depositing User: | Ummah Robithotul | ||||||||||||
| Date Deposited: | 22 Dec 2023 08:49 | ||||||||||||
| Last Modified: | 22 Dec 2023 08:49 | ||||||||||||
| URI: | http://repository.upnjatim.ac.id/id/eprint/19125 |
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