SUKENDAH, SUKENDAH and RAMDAN, HIDAYAT and BAKTI, WISNU WIDJAJANI (2020) Direct and indirect plantlet regeneration from explants of Albizia (Albizzia falcataria). Direct and indirect plantlet regeneration from explants of Albizia (Albizzia falcataria), 14. pp. 7211-7216. ISSN 7221-7216
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Abstract
Background: The research described here developed plantlets through direct and indirect organogenesis of selected explants. Materials and Methods: Sterile seeds were cultured in hormone-free Murashige & Skoog (MS) medium to generate explants. Nodal segments were cultured in MS basal medium for direct plantlet regeneration. Indirect plantlet regeneration through callus formation was done by culturing leaf, stem, and root explants. Explants from seeds were sterilized in 30% sodium hypochlorite for 20 minutes, 70% alcohol for 5 minutes, 20% sodium hypochlorite for 20 minutes, and 5% sodium hypochlorite. Results: The best concentration of BAP for direct plantlet regeneration was 2 mgL-1. Callus formation could be induced from the stem, leaf, and root explants; however, the largest amount of morphogenic callus was formed from stem explants. Albizia morphogenic callus had a friable and nodular structure, compact, shiny, with a yellowish color. This morphogenic callus regenerated into plantlets. Conclusions: Complete plantlets from direct and indirect in vitro regeneration were ready for acclimatization.
| Item Type: | Article |
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| Subjects: | S Agriculture > S Agriculture (General) > S590-599.9 Soils. Soil science |
| Divisions: | Faculty of Agriculture > Departement of Agritechnology |
| Depositing User: | Prof. Dr. Sukendah Sukendah |
| Date Deposited: | 02 May 2023 00:33 |
| Last Modified: | 02 May 2023 00:33 |
| URI: | http://repository.upnjatim.ac.id/id/eprint/13135 |
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