Sukendah, Sukendah and Makhziah, Makhziah and Witjaksono, Witjaksono (2016) Zygotic Embryo Excision and Somatic Embryogenesis Propagation of Kopyor Coconut. Zygotic Embryo Excision and Somatic Embryogenesis Propagation of Kopyor Coconut, 41 (1). ---. ISSN 0115-463x
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2016b-Volume 41(1) Philippine Journal Crop Science.pdf Download (9MB) | Preview |
Abstract
Propagation of kopyor coconut can be done only by in vitro culture. However, the available in vitro methods produce only one plantlet per zygotic embryo cultured. This paper discusses two in vitro methods to increase production of plantlets and seedlings of kopyor coconut, (1) the zygotic embryo excision and (2) somatic embryogenesis. Half of the embryonic explants were excised from freshly isolated embryo of the kopyor coconut fruit and inoculated in Eeuwens solid medium containing 60 g L-1 sucrose, 2.5 g L-1 activated charcoal, and 2.5-7.5 mg L-1 BAP. Embryogenic calli were induced from longitudinally or horizontally excised zygotic embryo explants and cultured on Eeuwens media containing 2,4-D and organic addenda. Observation was done on the percentage of regenerated plantlet, percentage of embryogenic calli, number of somatic embryos, plantlet formation, and morphological analysis. The halved embryo explants regenerated plantlet and shoot up to 83%, while the halved germinating embryo regenerated plantlet up to 100%. BAP improved shoot growth but not root growth and the best was 5 mg L-1. For somatic embryogensis, the addition of 2,4-D at 50-125 µM was required for induction of embryogenic culture. Addition of organic addenda such as casein hydrolysate, liquid coconut endosperm, and especially amino acid improved the induction of embryogenic culture. Morphological study demonstrated that somatic embryogenesis and the subsequent development of the somatic embryos were accompanied by the development of abnormal structures such as rootless shoot, shoot less root, multiple shoots, and non-developed structure. Nonetheless, plant has been regenerated from this process and in that case somatic embryos resembled the zygotic counterparts.
| Item Type: | Article |
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| Subjects: | S Agriculture > S Agriculture (General) > S590-599.9 Soils. Soil science |
| Divisions: | Faculty of Agriculture > Departement of Agritechnology |
| Depositing User: | Prof. Dr. Sukendah Sukendah |
| Date Deposited: | 02 May 2023 00:32 |
| Last Modified: | 02 May 2023 00:32 |
| URI: | http://repository.upnjatim.ac.id/id/eprint/13120 |
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