Efektivitas Metabolit Sekunder Jamur Trichoderma sp. Terhadap Ketahanan Jamur Fusarium sp. pada Tanaman Terung (Solanum melongena L.)

Hasanah, Uswatun (2023) Efektivitas Metabolit Sekunder Jamur Trichoderma sp. Terhadap Ketahanan Jamur Fusarium sp. pada Tanaman Terung (Solanum melongena L.). Undergraduate thesis, UPN Veteran Jawa Timur.

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Abstract

Eggplant (Solanum melongena L) is a vegetable plant belonging to the Solanaceae family. The demand for eggplant continues to increase in line with population growth, so eggplant production needs to be increased. According to FAO data (2012), Indonesia's eggplant production ranks sixth in the world with a production value of 518,827 tons, much lower than China and India. The low production rate is due to pathogen attack on plants. One of them is fusarium wilt caused by the pathogen Fusarium sp. which causes losses and crop failures of up to 50%. The use of secondary metabolites of Trichoderma sp. as an option for antagonist testing because it contains saponins, terpenoids, and alkaloids which are antimicrobial in nature and have the potential to be bioactive in controlling plant pathogens such as Fusarium sp. This research was conducted to determine the ability of secondary metabolites of Trichoderma sp. in inhibiting growth and suppressing the development of wilt disease in eggplant caused by the fungus Fusarium sp. This research was conducted from October to December 2022 in vitro at the Plant Health Laboratory and in vivo at the Green House of the Faculty of Agriculture, UPN "Veteran" East Java. The experimental design used a single factor Completely Randomized Design (CRD) with 5 treatments namely positive control, negative control, and secondary metabolite concentrations (10%, 20%, and 30%). So that the number of repetitions was 4 times with a total trial of 20 units. In vivo testing used 5 treatments, namely positive control and concentration of secondary metabolites. With a total of 4 replications, each replicate used 5 plant samples for each unit so that a total of 100 eggplant plants. This study used the antagonist test (dual culture method) in vitro. In vitro observations were carried out for 7 days with observation parameters, namely the percentage of inhibition and morphological observations after the antagonist test. In vivo observations were carried out once a week for 60 days with parameters of incubation period, plant growth (plant height and number of leaves), and disease intensity. The results showed that the secondary metabolites produced by Trichoderma sp. capable of inhibiting the growth of the fungus Fusarium sp. in vitro with a percentage of 26.47% at a concentration of 30% and causes abnormal growth of hyphae. In vivo secondary metabolites are able to inhibit the development of fusarium wilt well.

Item Type: Thesis (Undergraduate)
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorPurnawati, ArikaNIP196504221990032001arika_p@upnjatim.ac.id
Thesis advisorNirwanto, HerryNIP196206251991031003herry_n@upnjatim.ac.id
Subjects: S Agriculture > SB Plant culture > SB599-990.5 Pests and Diseases
Divisions: Faculty of Agriculture > Departement of Agritechnology
Depositing User: Uswatun Hasanah Mayar
Date Deposited: 30 May 2023 03:05
Last Modified: 30 May 2023 03:05
URI: http://repository.upnjatim.ac.id/id/eprint/14058

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